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bioRxiv · 10.1101/2023.05.20.541600

Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation

Stephens, D. C.·Mungai, M.·Crabtree, A.·Beasley, H. K.·Garza-Lopez, E.·Vang, L.·Neikirk, K.·Vue, Z.·Vue, N.·Marshall, A. G.·Turner, K.·Shao, J.-q.·Sarker, B.·Murray, S. A.·Gaddy, J. A.·Davis, J.·Damo, S. M.·Hinton, A.
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Abstract

Isolation of skeletal muscles allows for the exploration of many complex diseases. Fibroblasts and myoblast play important roles in skeletal muscle morphology and function. However, skeletal muscles are complex and made up of many cellular populations and validation of these populations is highly important. Therefore, in this article, we discuss a comprehensive method to isolate mice skeletal muscle, create satellite cells for tissue culture, and use immunofluorescence to validate our approach. Graphical Abstract O_FIG O_LINKSMALLFIG WIDTH=196 HEIGHT=200 SRC="FIGDIR/small/541600v2_ufig1.gif" ALT="Figure 1"> View larger version (45K): org.highwire.dtl.DTLVardef@165b34dorg.highwire.dtl.DTLVardef@1de9976org.highwire.dtl.DTLVardef@12d3cfeorg.highwire.dtl.DTLVardef@ff660c_HPS_FORMAT_FIGEXP M_FIG C_FIG

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Stephens, D. C., Mungai, M., Crabtree, A., Beasley, H. K., Garza-Lopez, E., Vang, L., Neikirk, K., Vue, Z., Vue, N., Marshall, A. G., Turner, K., Shao, J.-q., Sarker, B., Murray, S. A., Gaddy, J. A., Davis, J., Damo, S. M., Hinton, A.. 2023-05-22. Components of Isolated Skeletal Muscle Differentiated Through Antibody Validation. https://doi.org/10.1101/2023.05.20.541600

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