Thioflavin T In-gel Stain to Study Protein Misfolding in Frozen Tissue Specimens

Oldam, J.; Tchernyshyov, I.; Van Eyk, J.; Troncoso, J.; Glabe, C. G.; Agnetti, G.

doi:10.1101/2023.05.12.540528

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bioRxiv · 10.1101/2023.05.12.540528

Thioflavin T In-gel Stain to Study Protein Misfolding in Frozen Tissue Specimens

Oldam, J.·Tchernyshyov, I.·Van Eyk, J.·Troncoso, J.·Glabe, C. G.·Agnetti, G.

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Abstract

There are limited options to quantify and characterize amyloid species from biological samples in a simple fashion. Thioflavin T (ThT) has now been used for decades to stain amyloid fibrils but to our knowledge we were the first to use it in-gel. Thioflavin T in-gel stain is convenient as it is fast, inexpensive, available to most labs, compatible with other fluorescent stains and downstream analyses such as mass spectrometry (MS).

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Oldam, J., Tchernyshyov, I., Van Eyk, J., Troncoso, J., Glabe, C. G., Agnetti, G.. 2023-05-12. Thioflavin T In-gel Stain to Study Protein Misfolding in Frozen Tissue Specimens. https://doi.org/10.1101/2023.05.12.540528

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