Foxj1 controls olfactory ciliogenesis and differentiation program of the olfactory sensory neurons

Rayamajhi, D.; Ege, M.; Ukhanov, K.; Ringers, C.; Zhang, Y.; Jeong, I.; D'Gama, P. P.; Li, S. S.; Cosacak, M. I.; Kizil, C.; Park, H.-C.; Yaksi, E.; Martens, J. R.; Brody, S. L.; Jurisch-Yaksi, N.; Roy, S.

doi:10.1101/2023.05.10.540158

bioRxiv · 10.1101/2023.05.10.540158

Foxj1 controls olfactory ciliogenesis and differentiation program of the olfactory sensory neurons

Rayamajhi, D.·Ege, M.·Ukhanov, K.·Ringers, C.·Zhang, Y.·Jeong, I.·D'Gama, P. P.·Li, S. S.·Cosacak, M. I.·Kizil, C.·Park, H.-C.·Yaksi, E.·Martens, J. R.·Brody, S. L.·Jurisch-Yaksi, N.·Roy, S.

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Abstract

In vertebrates, olfactory receptors localize on multiple cilia elaborated on dendritic knobs of olfactory sensory neurons (OSNs). Although olfactory cilia dysfunction can cause anosmia, how their differentiation is programmed at the transcriptional level has remained largely unexplored. We discovered in zebrafish and mice that Foxj1, a forkhead domain-containing transcription factor linked with motile cilia biogenesis, is expressed in OSNs and required for olfactory epithelium (OE) formation. In keeping with the immotile nature of olfactory cilia, we observed that ciliary motility genes are repressed in zebrafish, mouse, and human OSNs. Strikingly, we also found that besides ciliogenesis, Foxj1 controls the differentiation of the OSNs themselves by regulating their cell type-specific gene expression, such as that of olfactory marker protein (omp) involved in odor-evoked signal transduction. In line with this, response to bile acid, an odor detected by OMP-positive OSNs, was significantly diminished in foxj1 mutant zebrafish. Taken together, our findings establish how the canonical Foxj1-mediated motile ciliogenic transcriptional program has been repurposed for the biogenesis of immotile olfactory cilia and for development of the OSNs.

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Rayamajhi, D., Ege, M., Ukhanov, K., Ringers, C., Zhang, Y., Jeong, I., D'Gama, P. P., Li, S. S., Cosacak, M. I., Kizil, C., Park, H.-C., Yaksi, E., Martens, J. R., Brody, S. L., Jurisch-Yaksi, N., Roy, S.. 2023-05-10. Foxj1 controls olfactory ciliogenesis and differentiation program of the olfactory sensory neurons. https://doi.org/10.1101/2023.05.10.540158

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