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bioRxiv · 10.1101/2023.05.08.539886

Transition from Transient DNA Rereplication to Inherited Gene Amplification Following Prolonged Environmental Stress

Wright, G. M.·Menzel, J.·Tatman, P. D.·Black, J. C.
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Abstract

Cells require the ability to adapt to changing environmental conditions, however, it is unclear how these changes elicit stable permanent changes in genomes. We demonstrate that, in response to environmental metal exposure, the metallothionein (MT) locus undergoes DNA rereplication generating transient site-specific gene amplifications (TSSGs). Chronic metal exposure allows transition from MT TSSG to inherited MT gene amplification through homologous recombination within and outside of the MT locus. DNA rereplication of the MT locus is suppressed by H3K27me3 and EZH2. Long-term ablation of EZH2 activity eventually leads to integration and inheritance of MT gene amplifications without the selective pressure of metal exposure. The rereplication and inheritance of MT gene amplification is an evolutionarily conserved response to environmental metal from yeast to human. Our results describe a new paradigm for adaptation to environmental stress where targeted, transient DNA rereplication precedes stable inherited gene amplification.

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Wright, G. M., Menzel, J., Tatman, P. D., Black, J. C.. 2023-05-09. Transition from Transient DNA Rereplication to Inherited Gene Amplification Following Prolonged Environmental Stress. https://doi.org/10.1101/2023.05.08.539886

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