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bioRxiv · 10.1101/2023.05.06.539685

Lipid modification of DNA nanocages enhances cellular uptake, migration, and in vivo uptake

Kansara, K.·Singh, R.·Yadav, P.·Kumar, A.·Bhatia, D.
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Abstract

The extraordinary self-assembling nature of DNA nanostructures and high functionality enables the formulation of DNA nanostructures with multiple chemical and biological molecules. How the whole organisms in native as well as modified; and how stable they are inside take up exactly these modified DNA nanostructures the organisms still remains to be explored. Here we report the fabrication and evaluation of a new conjugate of a cationic lipid, N-[one-(two, 3-dioleyloxy) propyl]-N, N, N-trimethylammonium chloride (DOTMA) and DNA tetrahedron nanostructure (TdN) for the enhanced uptake, stability, bioimaging, and biotherapeutics in cells and zebrafish (Danio rerio) eleuthero embryos as a model organism. We summarise the enhanced uptake potential of TdN-DOTMA conjugate for futuristic biomedical applications such as drug delivery, bioimaging, biosensing, and therapeutics.

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Kansara, K., Singh, R., Yadav, P., Kumar, A., Bhatia, D.. 2023-05-06. Lipid modification of DNA nanocages enhances cellular uptake, migration, and in vivo uptake. https://doi.org/10.1101/2023.05.06.539685

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