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bioRxiv · 10.1101/2023.05.01.538903

Genomic survey maps differences in the molecular complement of vesicle formation machinery between Giardia intestinalis assemblages

Pipaliya, S. V.·Dacks, J. B.·Croxen, M. A.
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Abstract

Giardia intestinalis is a globally important microbial pathogen with considerable public health, agricultural, and economic burden. Genome sequencing and comparative analyses have elucidated Giardia intestinalis to be a taxonomically diverse species consisting of at least eight different sub-types (assemblages A-H) that can infect a great variety of animal hosts, including humans. The best studied of these are assemblages A and B which have a broad host range and have zoonotic transmissibility towards humans where clinical Giardiasis can range from asymptomatic to diarrheal disease. Epidemiological surveys as well as previous molecular investigations have pointed towards critical genomic level differences within numerous molecular pathways and families of parasite virulence factors within assemblage A and B isolates. In this study, we explored the necessary machinery for the formation of vesicles and cargo transport in 89 Canadian isolates of assemblage A and B Giardia intestinalis. There is considerable variability within the molecular complement of the endolysosomal ESCRT protein machinery, adaptor coat protein complexes, and ARF regulatory system. We report inter-assemblage, but no intra-assemblage variation within the trafficking systems examined. These include losses of subunits belonging to the ESCRTIII as well as novel lineage specific duplications in components of the COPII machinery, ARF1, and ARFGEF families (BIG and CYTH). Since assemblages A and B show differences in disease manifestation, our findings may well have clinical implications and even taxonomic, as the membrane trafficking system underpin parasite survival, pathogenesis, and propagation.

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Pipaliya, S. V., Dacks, J. B., Croxen, M. A.. 2023-05-01. Genomic survey maps differences in the molecular complement of vesicle formation machinery between Giardia intestinalis assemblages. https://doi.org/10.1101/2023.05.01.538903

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