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bioRxiv · 10.1101/2023.05.01.538808

Hemin-driven chromatin remodelling by atherosclerotic risk gene SMARCA4 switches human blood-derived macrophages from leukocyte disposal to erythrocyte disposal

Cave, L.·Lodge, K. M.·Chiappo, D.·Sinha, S.·Chugtai, F.·Tsao, A.·Mason, J. C.·Haskard, D. O. J.·Humphries, S. E.·Boyle, J. J.

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Abstract

BackgroundPutative genetic risk loci for atherosclerotic vascular disease include SMARCA4, a chromatin remodeling gene important for gene activation. Its causal role in atherosclerosis has been uncertain. Intraplaque hemorrhage (IPH) is a late event in atherosclerosis that is linked to plaque destabilisation and increased inflammation. IPH is countered by Mhem macrophages, which are directed by hemin-mediated induction of Heme Oxygenase 1 (HMOX1) via Activating Transcription Factor 1 (ATF1). Atf1 deficiency in vivo impairs hematoma clearance, promoting inflammation and oxidative stress. Like its homologue cyclic-adenosine monophosphate response element binding protein 1 (CREB1), ATF1 is normally cyclic-AMP activated. HypothesisHemin-directed chromatin remodelling by SMARCA4 regulates specificity of ATF1 gene-binding, thereby switching between leukocyte disposal and erythrocyte disposal, contributing to its role in atherosclerosis. ResultsWe here show that SMARCA4 is genetically independent of the adjacent LDLR locus (p<0.05). In human blood-derived macrophages, hemin triggered histone acetylation (H3K9Ac) and SMARCA4 recruitment in advance of p-ATF1 recruitment at the HMOX1 enhancer. si-RNA-mediated SMARCA4-knockdown suppressed p-ATF1 binding to HMOX1 but increased its binding to cyclic-AMP responsive genes FOS and NR4A2, with corresponding changes in mRNA levels. This functionally correlated with SMARCA4-knockdown switching hemin to mimic prostacyclin (PGI2), for induced genes and phagocytic disposal of leukocytes rather than erythrocytes. ConclusionsThese data establish SMARCA4 as an independent atherosclerosis risk gene and reveal a novel mechanism in which it switches between disposal of leukocytes or erythrocytes, with important clinical implications for atherosclerotic inflammation and intraplaque hemorrhage including treatment by histone deacetylase inhibitors.

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Cave, L., Lodge, K. M., Chiappo, D., Sinha, S., Chugtai, F., Tsao, A., Mason, J. C., Haskard, D. O. J., Humphries, S. E., Boyle, J. J.. 2023-05-01. Hemin-driven chromatin remodelling by atherosclerotic risk gene SMARCA4 switches human blood-derived macrophages from leukocyte disposal to erythrocyte disposal. https://doi.org/10.1101/2023.05.01.538808

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