Integrated Spatial Transcriptomic and Proteomic Analysis of Fresh Frozen Tissue Based on Stereo-seq

Liao, S.; Heng, Y.; Liu, W.; Xiang, J.; Ma, Y.; Chen, L.; Feng, X.; Jia, D.; Liang, D.; Huang, C.; Zhang, J.; Jian, M.; Su, K.; Li, M.; Loh, Y. H.; Chen, A.; Xu, X.

doi:10.1101/2023.04.28.538364

bioRxiv · 10.1101/2023.04.28.538364

Integrated Spatial Transcriptomic and Proteomic Analysis of Fresh Frozen Tissue Based on Stereo-seq

Liao, S.·Heng, Y.·Liu, W.·Xiang, J.·Ma, Y.·Chen, L.·Feng, X.·Jia, D.·Liang, D.·Huang, C.·Zhang, J.·Jian, M.·Su, K.·Li, M.·Loh, Y. H.·Chen, A.·Xu, X.

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Abstract

To simultaneously detect whole transcriptomes and protein markers on the same tissue section, we combined Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) and Stereo-seq to develop the Stereo-CITE-seq workflow. Here, we demonstrated that Stereo-CITE-seq can co-detect mRNAs and proteins in immune organs with high spatial resolution, reproducibility and accuracy.

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Liao, S., Heng, Y., Liu, W., Xiang, J., Ma, Y., Chen, L., Feng, X., Jia, D., Liang, D., Huang, C., Zhang, J., Jian, M., Su, K., Li, M., Loh, Y. H., Chen, A., Xu, X.. 2023-04-28. Integrated Spatial Transcriptomic and Proteomic Analysis of Fresh Frozen Tissue Based on Stereo-seq. https://doi.org/10.1101/2023.04.28.538364

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