Scalable, flexible carbon fiber electrode thread arrays for three-dimensional spatial profiling of neurochemical activity in deep brain structures of rodents

Xia, M.; Agca, B. N.; Yoshida, T.; Choi, J.; Amjad, U.; Bose, K.; Keren, N.; Zukerman, S.; Cima, M. J.; Graybiel, A. M.; Schwerdt, H. N.

doi:10.1101/2023.04.15.537033

bioRxiv · 10.1101/2023.04.15.537033

Scalable, flexible carbon fiber electrode thread arrays for three-dimensional spatial profiling of neurochemical activity in deep brain structures of rodents

Xia, M.·Agca, B. N.·Yoshida, T.·Choi, J.·Amjad, U.·Bose, K.·Keren, N.·Zukerman, S.·Cima, M. J.·Graybiel, A. M.·Schwerdt, H. N.

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Abstract

We developed a flexible "electrode-thread" array for recording dopamine neurochemical activity from a lateral distribution of subcortical targets (up to 16) transverse to the axis of insertion. Ultrathin ([~] 10 {micro}m diameter) carbon fiber (CF) electrode-threads (CFETs) are clustered into a tight bundle to introduce them into the brain from a single entry point. The individual CFETs splay laterally in deep brain tissue during insertion due to their innate flexibility. This spatial redistribution allows navigation of the CFETs towards deep brain targets spreading horizontally from the axis of insertion. Commercial "linear" arrays provide single entry insertion but only allow measurements along the axis of insertion. Horizontally configured neurochemical recording arrays inflict separate penetrations for each individual channel (i.e., electrode). We tested functional performance of our CFET arrays in vivo for recording dopamine neurochemical dynamics and for providing lateral spread to multiple distributed sites in the striatum of rats. Spatial spread was further characterized using agar brain phantoms to measure electrode deflection as a function of insertion depth. We also developed protocols to slice the embedded CFETs within fixed brain tissue using standard histology techniques. This method allowed extraction of the precise spatial coordinates of the implanted CFETs and their recording sites as integrated with immunohistochemical staining for surrounding anatomical, cytological, and protein expression labels. Neurochemical recording operations tested here can be integrated with already widely established capabilities of CF-based electrodes to record single neuron activity and local field potentials, to enable multi-modal recording functions. Our CFET array has the potential to unlock a wide range of applications, from uncovering the role of neuromodulators in synaptic plasticity, to addressing critical safety barriers in clinical translation towards diagnostic and adaptive treatment in Parkinsons disease and major mood disorders.

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Xia, M., Agca, B. N., Yoshida, T., Choi, J., Amjad, U., Bose, K., Keren, N., Zukerman, S., Cima, M. J., Graybiel, A. M., Schwerdt, H. N.. 2023-04-17. Scalable, flexible carbon fiber electrode thread arrays for three-dimensional spatial profiling of neurochemical activity in deep brain structures of rodents. https://doi.org/10.1101/2023.04.15.537033

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