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bioRxiv · 10.1101/2023.04.13.536811

An integrated single-cell transcriptome landscape of postnatal mouse hypothalamus

Junaid, M.·Choe, H. K.·Kondoh, K.·Lee, E. J.·Lim, S. B.
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Abstract

The neural stem cells (NSCs) in the hypothalamus are relatively more narrowly defined than in other neurogenic regions of the postnatal brain. By leveraging single-cell RNA sequencing (scRNA-seq) data, we generated an integrated reference dataset comprising 296,282 cells from postnatal hypothalamic regions and the adjacent region, bed nucleus of the stria terminalis (BNST), and identified 30 hypothalamic neuronal and non-neuronal cell populations. The analyses of their gene expression pattern, and specific differentiation trajectories reveal the presence of NSCs and intermediate progenitor cells (IPCs) that show a continuum of activation and differentiation processes in the hypothalamus after birth. Through comparative analyses of the integrated dataset with our lab-generated Connect-seq data obtained from the whole hypothalamus, we further assessed the technical validity of the dataset presented in this study. Our large-scale unified scRNA-seq dataset with harmonized cell-level metadata can serve as a valuable resource for investigating cell type-specific gene expression and cellular differentiation trajectories in the postnatal mouse hypothalamus.

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Junaid, M., Choe, H. K., Kondoh, K., Lee, E. J., Lim, S. B.. 2023-04-14. An integrated single-cell transcriptome landscape of postnatal mouse hypothalamus. https://doi.org/10.1101/2023.04.13.536811

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