SpatialCTD: a large-scale TME spatial transcriptomic dataset to evaluate cell type deconvolution for immuno-oncology

Ding, J.; Venegas, J.; Lu, Q.; Wang, Y.; Wu, L.; Jin, W.; Wen, H.; Liu, R.; Tang, W.; Li, Z.; Zuo, W.; Chang, Y.; Lei, Y.; Danaher, P.; Xie, Y.; Tang, J.

doi:10.1101/2023.04.11.536333

bioRxiv · 10.1101/2023.04.11.536333

SpatialCTD: a large-scale TME spatial transcriptomic dataset to evaluate cell type deconvolution for immuno-oncology

Ding, J.·Venegas, J.·Lu, Q.·Wang, Y.·Wu, L.·Jin, W.·Wen, H.·Liu, R.·Tang, W.·Li, Z.·Zuo, W.·Chang, Y.·Lei, Y.·Danaher, P.·Xie, Y.·Tang, J.

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Abstract

Recent technological advancements have enabled spatially resolved transcriptomic profiling but at multi-cellular resolution. The task of cell type deconvolution has been introduced to disentangle discrete cell types from such multi-cellular spots. However, existing datasets for cell type deconvolution are limited in scale, predominantly encompassing data on mice, and are not designed for human immuno-oncology. In order to overcome these limitations and promote comprehensive investigation of cell type deconvolution for human immuno-oncology, we introduce a large-scale spatial transcriptomic dataset named SO_SCPLOWPATIALC_SCPLOWCTD, encompassing 1.8 million cells from the human tumor microenvironment across the lung, kidney, and liver. Distinct from existing approaches that primarily depend on single-cell RNA sequencing data as a reference without incorporating spatial information, we introduce Graph Neural Network-based method (i.e., GNNDO_SCPLOWECONVOLVERC_SCPLOW) that effectively utilize the spatial information from reference samples, and extensive experiments show that GNNDO_SCPLOWECONVOLVERC_SCPLOW often outperforms existing state-of-the-art methods by a substantial margin, without requiring single-cell RNA-seq data. To enable comprehensive evaluations on spatial transcriptomics data from flexible protocols, we provide an online tool capable of converting spatial transcriptomic data from other platforms (e.g., 10x Visium, MERFISH and sci-Space) into pseudo spots, featuring adjustable spot size. The SO_SCPLOWPATIALC_SCPLOWCTD dataset and GNNDO_SCPLOWECONVOLVERC_SCPLOW implementation are available at https://github.com/OmicsML/SpatialCTD, and the online converter tool can be accessed at https://omicsml.github.io/SpatialCTD/.

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Ding, J., Venegas, J., Lu, Q., Wang, Y., Wu, L., Jin, W., Wen, H., Liu, R., Tang, W., Li, Z., Zuo, W., Chang, Y., Lei, Y., Danaher, P., Xie, Y., Tang, J.. 2023-04-12. SpatialCTD: a large-scale TME spatial transcriptomic dataset to evaluate cell type deconvolution for immuno-oncology. https://doi.org/10.1101/2023.04.11.536333

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