Estimating heritability explained by local ancestry and evaluating stratification bias in admixture mapping from summary statistics

Chan, T. F.; Rui, X.; Conti, D. V.; Fornage, M.; Graff, M.; Haessler, J.; Haiman, C.; Highland, H. M.; Jung, S. Y.; Kenny, E.; Kooperberg, C.; Le Marchland, L.; North, K. E.; Tao, R.; Wojcik, G.; Gignoux, C. R.; PAGE Consortium,; Chiang, C. W.; Mancuso, N.

doi:10.1101/2023.04.10.536252

bioRxiv · 10.1101/2023.04.10.536252

Estimating heritability explained by local ancestry and evaluating stratification bias in admixture mapping from summary statistics

Chan, T. F.·Rui, X.·Conti, D. V.·Fornage, M.·Graff, M.·Haessler, J.·Haiman, C.·Highland, H. M.·Jung, S. Y.·Kenny, E.·Kooperberg, C.·Le Marchland, L.·North, K. E.·Tao, R.·Wojcik, G.·Gignoux, C. R.·PAGE Consortium,·Chiang, C. W.·Mancuso, N.

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Abstract

The heritability explained by local ancestry markers in an admixed population [Formula] provides crucial insight into the genetic architecture of a complex disease or trait. Estimation of [Formula] can be susceptible to biases due to population structure in ancestral populations. Here, we present a novel approach, Heritability estimation from Admixture Mapping Summary STAtistics (HAMSTA), which uses summary statistics from admixture mapping to infer heritability explained by local ancestry while adjusting for biases due to ancestral stratification. Through extensive simulations, we demonstrate that HAMSTA [Formula] estimates are approximately unbiased and are robust to ancestral stratification compared to existing approaches. In the presence of ancestral stratification, we show a HAMSTA-derived sampling scheme provides a calibrated family-wise error rate (FWER) of [~]5% for admixture mapping, unlike existing FWER estimation approaches. We apply HAMSTA to 20 quantitative phenotypes of up to 15,988 self-reported African American individuals in the Population Architecture using Genomics and Epidemiology (PAGE) study. We observe [Formula] in the 20 phenotypes range from 0.0025 to 0.033 (mean [Formula]), which translates to [Formula] ranging from 0.062 to 0.85 (mean [Formula]). Across these phenotypes we find little evidence of inflation due to ancestral population stratification in current admixture mapping studies (mean inflation factor of 0.99 +/-0.001). Overall, HAMSTA provides a fast and powerful approach to estimate genome-wide heritability and evaluate biases in test statistics of admixture mapping studies.

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Chan, T. F., Rui, X., Conti, D. V., Fornage, M., Graff, M., Haessler, J., Haiman, C., Highland, H. M., Jung, S. Y., Kenny, E., Kooperberg, C., Le Marchland, L., North, K. E., Tao, R., Wojcik, G., Gignoux, C. R., PAGE Consortium,, Chiang, C. W., Mancuso, N.. 2023-04-10. Estimating heritability explained by local ancestry and evaluating stratification bias in admixture mapping from summary statistics. https://doi.org/10.1101/2023.04.10.536252

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