Rats emit aversive 44-kHz ultrasonic vocalizations during prolonged Pavlovian fear conditioning

Abstract

Rats are believed to communicate their emotional state by emitting two distinct types of ultrasonic vocalizations. The first is long "22-kHz" vocalizations (>300 ms, <32 kHz) with constant frequency, signaling aversive states and the second, is short "50-kHz" calls (<150 ms, >32 kHz), often frequency-modulated, in appetitive situations. Here we describe aversive vocalizations emitted at a higher pitch by male Wistar and spontaneously hypertensive rats (SHR) in an intensified aversive state - prolonged fear conditioning. These calls, which we named "44-kHz" vocalizations, are long (>150 ms), generally at a constant frequency (usually within 35-50 kHz range) and have an overall spectrographic image similar to 22-kHz calls. Some 44-kHz vocalizations are comprised of both 22-kHz-like and 44-kHz-like elements. Furthermore, two separate clustering methods confirmed that these 44-kHz calls can be separated from other vocalizations. We observed 44-kHz calls to be associated with freezing behavior during fear conditioning training, during which they constituted up to 19.4% of all calls and most of them appeared next to each other forming uniform groups of vocalizations (bouts). We also show that some of rats responses to the playback of 44-kHz calls were more akin to that of aversive calls, e.g., heart rate changes, whereas other responses were at an intermediate level between aversive and appetitive calls. Our results suggest that rats have a wider vocal repertoire than previously believed, and current definitions of major call types may require reevaluation. We hope that future investigations of 44-kHz calls in rat models of human diseases will contribute to expanding our understanding and therapeutic strategies related to human psychiatric conditions.