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bioRxiv · 10.1101/2023.04.04.535522

Meta-analysis of gene activity (MAGA) contributions and correlation with gene expression, through GAGAM.

Martini, L.·Bardini, R.·Savino, A.·Di Carlo, S.
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Abstract

It is well-known how sequencing technologies propelled cellular biology research in the latest years, giving an incredible insight into the basic mechanisms of cells. Single-cell RNA sequencing is at the front in this field, with Single-cell ATAC sequencing supporting it and becoming more popular. In this regard, multi-modal technologies play a crucial role, allowing the possibility to perform the mentioned sequencing modalities simultaneously on the same cells. Yet, there still needs to be a clear and dedicated way to analyze this multi-modal data. One of the current methods is to calculate the Gene Activity Matrix, which summarizes the accessibility of the genes at the genomic level, to have a more direct link with the transcriptomic data. However, this concept is not well-defined, and it is unclear how various accessible regions impact the expression of the genes. Therefore, this work presents a meta-analysis of the Gene Activity matrix based on the Genomic-Annotated Gene Activity Matrix model, aiming to investigate the different influences of its contributions on the activity and their correlation with the expression. This allows having a better grasp on how the different functional regions of the genome affect not only the activity but also the expression of the genes.

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Martini, L., Bardini, R., Savino, A., Di Carlo, S.. 2023-04-06. Meta-analysis of gene activity (MAGA) contributions and correlation with gene expression, through GAGAM.. https://doi.org/10.1101/2023.04.04.535522

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