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bioRxiv · 10.1101/2023.04.01.535194

Circular RNAs in the human brain are tailored to neuron identity and neuropsychiatric disease

Dong, X.·Bai, Y.·Liao, Z.·Gritsch, D.·Liu, X.·Wang, T.·Borges-Monroy, R.·Ehrlich, A.·Serano, G. E.·Feany, M. B.·Beach, T. G.·Scherzer, C. R.
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Abstract

Little is known about circular RNAs (circRNAs) in specific brain cells and human neuropsychiatric disease. Here, we systematically identified over 11,039 circRNAs expressed in vulnerable dopamine and pyramidal neurons laser-captured from 190 human brains and non-neuronal cells using ultra-deep, total RNA sequencing. 1,526 and 3,308 circRNAs were custom-tailored to the cell identity of dopamine and pyramidal neurons and enriched in synapse pathways. 88% of Parkinsons and 80% of Alzheimers disease-associated genes produced circRNAs. circDNAJC6, produced from a juvenile-onset Parkinsons gene, was already dysregulated during prodromal, onset stages of common Parkinsons disease neuropathology. Globally, addiction-associated genes preferentially produced circRNAs in dopamine neurons, autism-associated genes in pyramidal neurons, and cancers in non-neuronal cells. This study shows that circular RNAs in the human brain are tailored to neuron identity and implicate circRNA- regulated synaptic specialization in neuropsychiatric diseases.

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Dong, X., Bai, Y., Liao, Z., Gritsch, D., Liu, X., Wang, T., Borges-Monroy, R., Ehrlich, A., Serano, G. E., Feany, M. B., Beach, T. G., Scherzer, C. R.. 2023-04-03. Circular RNAs in the human brain are tailored to neuron identity and neuropsychiatric disease. https://doi.org/10.1101/2023.04.01.535194

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