Increase in primary cilia number and length upon VDAC1 depletion contributes to attenuated proliferation of cancer cells

Dutta, A.; Halder, P.; Gayen, A.; Mukherjee, A.; Mukherjee, C.; Majumder, S.

doi:10.1101/2023.03.31.535181

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bioRxiv · 10.1101/2023.03.31.535181

Increase in primary cilia number and length upon VDAC1 depletion contributes to attenuated proliferation of cancer cells

Dutta, A.·Halder, P.·Gayen, A.·Mukherjee, A.·Mukherjee, C.·Majumder, S.

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Abstract

Primary cilia (PCs) that are present in most human cells and perform sensory function or signal transduction are lost in many solid tumors. Previously, we identified VDAC1, best known to regulate mitochondrial bioenergetics, to negatively regulate ciliogenesis. Here, we show that downregulation of VDAC1 in pancreatic cancer-derived Panc1 and glioblastoma-derived U-87 cells significantly increased ciliation. Those PCs were remarkably longer than the control cells. Such increased ciliation inhibited cell cycle, which contributed to reduced proliferation of these cells. VDAC1-depletion also led to longer PCs in quiescent RPE1 cells. Therefore, serum-induced PC disassembly was slower in VDAC1-depleted RPE1 cells. Overall, this study reiterates the importance of VDAC1 in modulating tumorigenesis, due to its novel role in regulating PC length and disassembly.

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Dutta, A., Halder, P., Gayen, A., Mukherjee, A., Mukherjee, C., Majumder, S.. 2023-04-02. Increase in primary cilia number and length upon VDAC1 depletion contributes to attenuated proliferation of cancer cells. https://doi.org/10.1101/2023.03.31.535181

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