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bioRxiv · 10.1101/2023.03.29.534741

Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation

Abstract

The CTP nucleotide is a key precursor of nucleic acids metabolism essential for DNA replication. De novo CTP production relies on CTP synthetases 1 and 2 (CTPS1 and 2) that catalyze the conversion of UTP into CTP. CTP synthetase activity is high in proliferating cells including cancer cells, however, the respective roles of CTPS1 and CTPS2 in cell proliferation are not known. By inactivation of CTPS1 and/or CTPS2 and complementation experiments, we showed that both CTPS1 and CTPS2 are differentially required for cell proliferation. CTPS1 was more efficient in promoting proliferation than CTPS2, in association with a higher intrinsic enzymatic activity that was more resistant to inhibition by 3-Deaza-uridine, an UTP analog. The contribution of CTPS2 to cell proliferation was modest when CTPS1 was expressed, but essential in absence of CTPS1. Public databases analysis of more than 1,000 inactivated cancer cell lines for CTPS1 or CTPS2 confirmed that cell growth is highly dependent of CTPS1 but less of CTPS2. Therefore, our results demonstrate that CTPS1 is the main contributor to cell proliferation.

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BibTeXRIS

Latour, S., Minet, N., Boschat, A.-C., Lane, R., Laughton, D., Beer, P., Asnagli, H., Soudais, C., Bourne, T., Fischer, A., Martin, E.. 2023-04-03. Differential roles of CTP synthetases CTPS1 and CTPS2 in cell proliferation. https://doi.org/10.1101/2023.03.29.534741

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