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bioRxiv · 10.1101/2023.03.29.534669

FADD- and RIPK3-mediated cell death ensures timely clearance of wound macrophages and promotes wound healing

Abstract

Cells of the monocyte/macrophage lineage are an integral component of the bodys innate ability to restore tissue function after injury. In parallel to mounting an inflammatory response, clearance of monocytes/macrophages from the wound site is critical to re-establish tissue functionality and integrity during the course of healing. The role of regulated cell death in macrophage clearance from damaged tissue and its implications for the outcome of the healing response is little understood. Here, we explored the role of macrophage-specific FADD-mediated cell death on Ripk3-/- background in a mechanical skin injury model in mice. We found that combined inhibition of RIPK3-mediated necroptosis and FADD-caspase-8-mediated apoptosis in macrophages profoundly delayed wound healing. Importantly, RIPK3 deficiency alone did not considerably alter the wound healing process and macrophage population dynamics, arguing that inhibition of FADD-caspase-8-dependent death of macrophages is primarily responsible for the delayed wound closure. Notably, TNF blockade reversed the accumulation of Ly6Chigh macrophages induced by combined deficiency of FADD and RIPK3, indicating a critical dual role of TNF-mediated pro-survival and cell death signalling, particularly in this highly pro-inflammatory macrophage subset. Our findings reveal a previously uncharacterized cross-talk of inflammatory and cell death signalling in macrophages in regulating repair processes in the skin.

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BibTeXRIS

Injarabian, L., Willenborg, S., Welcker, D., Pasparakis, M., Kashkar, H., Eming, S. A.. 2023-03-29. FADD- and RIPK3-mediated cell death ensures timely clearance of wound macrophages and promotes wound healing. https://doi.org/10.1101/2023.03.29.534669

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