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bioRxiv · 10.1101/2023.03.27.534392

β-actin is essential for structural integrity and physiological function of the retina

Vedula, P.·Fina, M. E.·Bell, B. A.·Nikonov, S. S.·Kashina, A.·Dong, D. W.
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Abstract

Lack of non-muscle {beta}-actin gene (Actb) leads to early embryonic lethality in mice, however mice with {beta}- to{gamma} -actin replacement develop normally and show no detectable phenotypes at young age. Here we investigated the effect of this replacement in the retina. During aging, these mice have accelerated de-generation of retinal structure and function, including elongated microvilli and defective mitochondria of retinal pigment epithelium (RPE), abnormally bulging photoreceptor outer segments (OS) accompanied by reduced transducin concentration and light sensitivity, and accumulation of autofluorescent microglia cells in the subretinal space between RPE and OS. These defects are accompanied by changes in the F-actin binding of several key actin interacting partners, including ezrin, myosin, talin, and vinculin known to play central roles in modulating actin cytoskeleton and cell adhesion and mediating the phagocytosis of OS. Our data show that {beta}-actin protein is essential for maintaining normal retinal structure and function.

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Vedula, P., Fina, M. E., Bell, B. A., Nikonov, S. S., Kashina, A., Dong, D. W.. 2023-03-27. β-actin is essential for structural integrity and physiological function of the retina. https://doi.org/10.1101/2023.03.27.534392

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