Three-dimensional reconstructions of mechanosensory end organs suggest a unifying mechanism underlying dynamic, light touch

Handler, A.; Zhang, Q.; Pang, S.; Nguyen, T. M.; Iskols, M.; Nolan-Tamariz, M.; Cattel, S.; Plumb, R.; Sanchez, B.; Ashjian, K.; Shotland, A.; Brown, B.; Kabeer, M.; Turecek, J.; Rankin, G.; Xiang, W.; Pavarino, E. C.; Africawala, N.; Santiago, C.; Lee, W.-C. A.; Xu, C. S.; Ginty, D. D.

doi:10.1101/2023.03.17.533188

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bioRxiv · 10.1101/2023.03.17.533188

Three-dimensional reconstructions of mechanosensory end organs suggest a unifying mechanism underlying dynamic, light touch

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Abstract

Specialized mechanosensory end organs within mammalian skin--hair follicle-associated lanceolate complexes, Meissner corpuscles, and Pacinian corpuscles--enable our perception of light, dynamic touch1. In each of these end organs, fast-conducting mechanically sensitive neurons, called A{beta} low-threshold mechanoreceptors (A{beta} LTMRs), associate with resident glial cells, known as terminal Schwann cells (TSCs) or lamellar cells, to form complex axon ending structures. Lanceolate-forming and corpuscle-innervating A{beta} LTMRs share a low threshold for mechanical activation, a rapidly adapting (RA) response to force indentation, and high sensitivity to dynamic stimuli1-6. How mechanical stimuli lead to activation of the requisite mechanotransduction channel Piezo27-15 and A{beta} RA-LTMR excitation across the morphologically dissimilar mechanosensory end organ structures is not understood. Here, we report the precise subcellular distribution of Piezo2 and high-resolution, isotropic 3D reconstructions of all three end organs formed by A{beta} RA-LTMRs determined by large volume enhanced Focused Ion Beam Scanning Electron Microscopy (FIB-SEM) imaging. We found that within each end organ, Piezo2 is enriched along the sensory axon membrane and is minimally or not expressed in TSCs and lamellar cells. We also observed a large number of small cytoplasmic protrusions enriched along the A{beta} RA-LTMR axon terminals associated with hair follicles, Meissner corpuscles, and Pacinian corpuscles. These axon protrusions reside within close proximity to axonal Piezo2, occasionally contain the channel, and often form adherens junctions with nearby non-neuronal cells. Our findings support a unified model for A{beta} RA-LTMR activation in which axon protrusions anchor A{beta} RA-LTMR axon terminals to specialized end organ cells, enabling mechanical stimuli to stretch the axon in hundreds to thousands of sites across an individual end organ and leading to activation of proximal Piezo2 channels and excitation of the neuron.

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Handler, A., Zhang, Q., Pang, S., Nguyen, T. M., Iskols, M., Nolan-Tamariz, M., Cattel, S., Plumb, R., Sanchez, B., Ashjian, K., Shotland, A., Brown, B., Kabeer, M., Turecek, J., Rankin, G., Xiang, W., Pavarino, E. C., Africawala, N., Santiago, C., Lee, W.-C. A., Xu, C. S., Ginty, D. D.. 2023-03-18. Three-dimensional reconstructions of mechanosensory end organs suggest a unifying mechanism underlying dynamic, light touch. https://doi.org/10.1101/2023.03.17.533188

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