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bioRxiv · 10.1101/2023.03.10.532097

Generating high quality reference genomes from field collected specimens by optimizing preservation

Minich, J. J.·Moore, M. L.·Allsing, N. A.·Murray, E. R.·Tran, L.·Michael, T. P.
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Abstract

Sample preservation challenges efforts to generate high-quality reference genomes or pangenomes for all 2 million+ plant and animal species. Here we show 95% ethanol protects against degradation for fish blood (22{degrees}C, [≤]6 weeks) and plant tissue (4{degrees}C, [≤]3 weeks). Using Nanopore, we assemble high quality reference genomes from three fish and two plant species (contig N50: 6.5-13.8Mb; BUSCO completeness: 94.4-99.2%; QV: 43.8 for M. esculenta).

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Minich, J. J., Moore, M. L., Allsing, N. A., Murray, E. R., Tran, L., Michael, T. P.. 2023-03-12. Generating high quality reference genomes from field collected specimens by optimizing preservation. https://doi.org/10.1101/2023.03.10.532097

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