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bioRxiv · 10.1101/2023.03.10.532006

Ketogenic diet prevents sucrose withdrawal-induced anxiety-like behaviour in male mice

Kumar, M.·Lal, R.·Bhatt, B.·Bishnoi, M.
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Abstract

Sugar bingeing has been shown to induce withdrawal effects when sugar is no longer available in the diet. In this study, we investigated the sex differences in sugar-addiction-like behaviour and the effects of the ketogenic diet on these outcomes. We used the two-bottle sucrose choice paradigm as a pre-clinical sucrose overeating and withdrawal model. Female mice consumed more sucrose than males when given free access to water and 10% sucrose for four weeks. One week of sucrose withdrawal after four weeks of consecutive sucrose overeating showed anxiety-like behaviour in male mice. However, the ketogenic diet did not affect sucrose overconsumption in males and females but prevented the sucrose withdrawal-induced anxiety-like behaviour in male mice with a concomitant increase in cFos mRNA in the prefrontal cortex. These findings provide evidence of sex differences in sucrose addiction-like behaviour and also indicate that a ketogenic diet may prevent sucrose withdrawal-induced anxiety-like behaviour in males. However, further research is needed to explore the underlying molecular mechanisms driving sex differences in sugar-addiction-like behaviour and the anxiolytic effects of the ketogenic diet.

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Kumar, M., Lal, R., Bhatt, B., Bishnoi, M.. 2023-03-10. Ketogenic diet prevents sucrose withdrawal-induced anxiety-like behaviour in male mice. https://doi.org/10.1101/2023.03.10.532006

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