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bioRxiv · 10.1101/2023.03.08.531834

Further evidences of an emerging stingless bee-yeast symbiosis

de Paula, G. T.·Melo, W. G. P.·de Castro, I.·Menezes, C.·Paludo, C. R.·Rosa, C. A.·Pupo, M. T.
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Abstract

Symbiotic interactions between microorganisms and social insects have been described as crucial for the maintenance of these multitrophic systems, as observed for the stingless bee Scaptotrigona depilis and the yeast Zygosaccharomyces sp. The larvae of S. depilis ingest fungal filaments of Zygosaccharomyces sp. to obtain ergosterol, which is the precursor for the biosynthesis of ecdysteroids that modulate insect metamorphosis. In this work we verified that nutritional fungal symbioses also occur in other species of stingless bees. We analyzed brood cell samples from 19 species of stingless bees collected in Brazil. The osmophilic yeast Zygosaccharomyces spp. was isolated from eight bee species, namely Scaptotrigona bipuctata, S. postica, S. tubiba, Tetragona clavipes, Melipona quadrifasciata, M. fasciculata, M. bicolor and Partamona helleri. These yeasts form pseudohyphae and also accumulate ergosterol in lipid droplets, similar to the pattern observed for S. depilis. The phylogenetic analyses including various Zygosaccharomyces revealed that strains isolated from the brood cells formed a branch separated from the previously described Zygosaccharomyces species, suggesting that they are new species of this genus and reinforcing the symbiotic interaction with the host insects. ImportanceBenefits exchanged in insect-fungus mutualisms include nutrition, protection, and dispersal. Fungal nutritional roles are well described for some eusocial insects, such as fungus growing ants and termites, but similar interaction in stingless bees was so far observed just in Scaptotrigona depilis. Here we expand the knowledge of yeast-bee symbiosis by analyzing the presence, cell morphologies, lipid accumulation and phylogenetic relationships of fungi isolated from brood cells and other locations of bee colonies. Zygosaccharomyces isolates were recovered from 42% of the bee species assessed, and probably represent new species showing pseudohyphae formation and lipid accumulation similar to S. depilis associated Zygosaccharomyces strains. The phylogenetic analyses suggested an evolutionary adaptation of Zygosaccharomyces spp. to the brood cell environment to provide nutritional benefits for the developing insect. Stingless bees play important ecosystem services, and our results raise the concern that fungicidal agents used in agriculture could disrupt this symbiosis, impacting bee health.

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de Paula, G. T., Melo, W. G. P., de Castro, I., Menezes, C., Paludo, C. R., Rosa, C. A., Pupo, M. T.. 2023-03-10. Further evidences of an emerging stingless bee-yeast symbiosis. https://doi.org/10.1101/2023.03.08.531834

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