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bioRxiv · 10.1101/2023.03.07.531644

Temporal landscape and translational regulation of A-to-I editing in mouse retina development

Yang, L.·Yi, L.·Yang, J.·Zhang, R.·Xie, Z.·Wang, H.
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Abstract

The significance of A-to-I RNA editing in neurological development is widely recognized, however, its influence on retina development remains to be thoroughly understood. In this study, we aimed to characterize the temporal landscape of A-to-I editing in mouse retina development using total RNA-seq and Ribosome profiling, with a specific emphasis on its effect on gene translation. Our findings revealed that the editing underwent plastic changes and distinct editing contents facilitated stage-specific functions. Further analysis showed a dynamic interplay between A-to-I editing and alternative splicing, with A-to-I editing having a significant impact on splicing efficiency and increasing the quantity of splicing events. A-to-I editing held the potential of enhancing the translatomes diversity, but this came at the expense of reduced translational efficiency. When coupled with splicing, it could produce a coordinated regulatory effect on translatome dynamics. Overall, our study presents a temporally resolved atlas of A-to-I editing, connecting its dynamic changes with the regulatory impact on splicing and translation.

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Yang, L., Yi, L., Yang, J., Zhang, R., Xie, Z., Wang, H.. 2023-03-08. Temporal landscape and translational regulation of A-to-I editing in mouse retina development. https://doi.org/10.1101/2023.03.07.531644

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