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bioRxiv · 10.1101/2023.03.01.530686

SnapHiC-G: identifying long-range enhancer-promoter interactions from single-cell Hi-C data via a global background model

Liu, W.·Zhong, W.·Giusti-Rodriguez, P.·Wang, G. W.·Hu, M.·Li, Y.
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Abstract

Harnessing the power of single-cell genomics technologies, single-cell Hi-C (scHi-C) and its derived technologies provide powerful tools to measure spatial proximity between regulatory elements and their target genes in individual cells. Using a global background model, we propose SnapHiC-G, a computational method to identify long-range enhancer-promoter interactions from scHi-C data. We applied SnapHiC-G to scHi-C datasets generated from mouse embryonic stem cells and human brain cortical cells and demonstrated that SnapHiC-G achieved high sensitivity in identifying long-range enhancer-promoter interactions. Moreover, SnapHiC-G can identify putative target genes for non-coding GWAS variants, and the genetic heritability of neuropsychiatric diseases is enriched for single nucleotide polymorphisms (SNPs) within SnapHiC-G-identified interactions in a cell-type-specific manner. In sum, SnapHiC-G is a powerful tool for characterizing cell-type-specific enhancer-promoter interactions from complex tissues and can facilitate the discovery of chromatin interactions important for gene regulation in biologically relevant cell types.

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Liu, W., Zhong, W., Giusti-Rodriguez, P., Wang, G. W., Hu, M., Li, Y.. 2023-03-02. SnapHiC-G: identifying long-range enhancer-promoter interactions from single-cell Hi-C data via a global background model. https://doi.org/10.1101/2023.03.01.530686

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