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bioRxiv · 10.1101/2023.02.25.529923

The axon guidance cue SEMA3A promotes the aggressive phenotype of basal-like PDAC

Abstract

Pancreatic ductal adenocarcinoma (PDAC) is a lethal disease with few available therapeutic options. Two transcriptional cancer cell states have been consistently reported in PDAC, with the basal-like/squamous phenotype displaying a more aggressive biological behavior. Genetic and epigenetic dysregulation of the axon guidance pathway are common in PDAC, yet our understanding of its biological relevance is limited. Here, we investigated the functional role of the axon guidance cue SEMA3A in sustaining the progression of PDAC. We integrated available transcriptomic datasets of human PDAC with in situ hybridization analyses of patients tissues to find that SEMA3A is expressed by stromal cells and selectively enriched in epithelial cells of the basal-like/squamous subtype. We found that both cell-intrinsic and cell extrinsic factors instructing the basal-like/squamous subtype induce expression of SEMA3A in PDAC cells. In vitro, SEMA3A promoted cell migration as well as anoikis resistance. At molecular level, these phenotypes were associated with increased FAK signaling and enrichment of gene programs related to cytoskeleton remodeling. Accordingly, SEMA3A provided mouse PDAC cells with greater metastatic competence. In mouse orthotopic allografts, SEMA3A remodeled the TME by favoring infiltration of tumor-associated macrophages and exclusion of T cells. Mechanistically, SEMA3A functioned as chemoattractant for macrophages and favored their polarization towards an M2-like phenotype. In SEMA3Ahigh tumors, depletion of macrophages resulted in greater intratumor infiltration by CD8+ T cells and increased sensitivity of these tumors to chemotherapy. Overall, we show that SEMA3A contributes to the malignant phenotype of basal-like PDAC.

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BibTeXRIS

Lupo, F., Pezzini, F., Fiorini, E., Adamo, A., Veghini, L., Bevere, M., Frusteri, C., Delfino, P., D'Agosto, S., Andreani, S., Piro, G., Malinova, A., De Sanctis, F., Pasini, D., Lawlor, R. T., Hwang, C.-i., Carbone, C., Amelio, I., Bailey, P., Bronte, V., Tuveson, D., Scarpa, A., Ugel, S., Corbo, V.. 2023-02-26. The axon guidance cue SEMA3A promotes the aggressive phenotype of basal-like PDAC. https://doi.org/10.1101/2023.02.25.529923

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