SP2G: an imaging and analysis pipeline revealing the inter and intra-patient migratory diversity of glioblastoma

Crestani, M.; Kakogiannos, N.; Iannelli, F.; Dini, T.; Maderna, C.; Giannotta, M.; Pelicci, G.; Maiuri, P.; Monzo, P.; Gauthier, N. C.

doi:10.1101/2023.02.24.529679

bioRxiv · 10.1101/2023.02.24.529679

SP2G: an imaging and analysis pipeline revealing the inter and intra-patient migratory diversity of glioblastoma

Crestani, M.·Kakogiannos, N.·Iannelli, F.·Dini, T.·Maderna, C.·Giannotta, M.·Pelicci, G.·Maiuri, P.·Monzo, P.·Gauthier, N. C.

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Abstract

Glioblastomas are heterogeneous, primary brain tumors hiding several sub-populations. Patient-derived xenografts are considered gold-standards to study glioblastoma invasion. However, they present many disadvantages, including time consumption, complex standardization, high cost. To counteract these issues and rapidly identify the most invasive sub-populations, we developed an in vivo mimicry platform named SP2G (SPheroid SPreading on Grids). Live imaging of tumor-derived spheroids spreading on gridded micro patterns mimicking the brain vasculature recapitulated 3D motility features observed in brain or 3D matrices. Using patient-derived samples coupled with a semi-automated macro suite, SP2G easily characterized and sorted differences in cell migration and motility modes. Moreover, SP2G exposed the hidden intra-patient heterogeneity in cell motility that correlated molecularly to specific integrins. Thus, SP2G constitute a versatile and potentially pan-cancer workflow to identify the diverse invasive tumor sub-populations in patient-derived specimens. SP2G includes an integrative tool, available as open-source Fiji macro suite, for therapeutic evaluations at single patient level. TeaserCracking the inter and intra-patient diversity in Glioblastoma migration profiles

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Crestani, M., Kakogiannos, N., Iannelli, F., Dini, T., Maderna, C., Giannotta, M., Pelicci, G., Maiuri, P., Monzo, P., Gauthier, N. C.. 2023-02-24. SP2G: an imaging and analysis pipeline revealing the inter and intra-patient migratory diversity of glioblastoma. https://doi.org/10.1101/2023.02.24.529679

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