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bioRxiv · 10.1101/2023.02.17.528965

NSD1 deposits histone H3 lysine 36 dimethylation to pattern non-CG DNA methylation in neurons

Hamagami, N.·Wu, D. Y.·Clemens, A. W.·Nettles, S. A.·Gabel, H. W.
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Abstract

During postnatal development the DNA methyltransferase DNMT3A deposits high levels of non-CG cytosine methylation in neurons. This unique methylation is critical for transcriptional regulation in the mature mammalian brain, and loss of this mark is implicated in DNMT3A-associated neurodevelopmental disorders (NDDs). The mechanisms determining genomic non-CG methylation profiles are not well defined however, and it is unknown if this pathway is disrupted in additional NDDs. Here we show that genome topology and gene expression converge to shape histone H3 lysine 36 dimethylation (H3K36me2) profiles, which in turn recruit DNMT3A and pattern neuronal non-CG methylation. We show that NSD1, the H3K36 methyltransferase mutated in the NDD, Sotos syndrome, is required for megabase-scale patterning of H3K36me2 and non-CG methylation in neurons. We find that brain-specific deletion of NSD1 causes alterations in DNA methylation that overlap with models of DNMT3A disorders and define convergent disruption in the expression of key neuronal genes in these models that may contribute to shared phenotypes in NSD1- and DNMT3A-associated NDD. Our findings indicate that H3K36me2 deposited by NSD1 is an important determinant of neuronal non-CG DNA methylation and implicates disruption of this methylation in Sotos syndrome. HighlightsO_LITopology-associated DNA methylation and gene expression independently contribute to neuronal gene body and enhancer non-CG DNA methylation patterns. C_LIO_LITopology-associated H3K36me2 patterns and local enrichment of H3K4 methylation impact deposition of non-CG methylation by DNMT3A. C_LIO_LIDisruption of NSD1 in vivo leads to alterations in H3K36me2, DNA methylation, and gene expression that overlap with models of DNMT3A disorders. C_LI

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Hamagami, N., Wu, D. Y., Clemens, A. W., Nettles, S. A., Gabel, H. W.. 2023-02-17. NSD1 deposits histone H3 lysine 36 dimethylation to pattern non-CG DNA methylation in neurons. https://doi.org/10.1101/2023.02.17.528965

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