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bioRxiv · 10.1101/2023.02.15.528366

Differentiation state and culture conditions impact neural stem/progenitor cell-derived extracellular vesicle bioactivity

Dutta, D.·Pirolli, N. H.·Levy, D.·Tsao, J.·Seecharan, N.·Xu, X.·Wang, Z.·Jia, X.·Jay, S. M.
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Abstract

Extracellular vesicles (EVs) derived from neural progenitor/stem cells (NPSCs) have shown promising efficacy in a variety of preclinical models. However, NPSCs lack critical neuroregenerative functionality such as myelinating capacity. Further, culture conditions used in NPSC EV production lack standardization and identification of optimal conditions for NPSC EV neurogenic bioactivity. Here, we assessed whether further differentiated oligodendrocyte precursor cells (OPCs) and immature oligodendrocytes (iOLs) that give rise to mature myelinating oligodendrocytes could yield EVs with neurotherapeutic properties comparable or superior to those from NPSCs as well as mesenchymal stromal cells (MSCs), as MSC EVs are also commonly reported to have neurotherapeutic activity. We additionally examined the effects of four different extracellular matrix (ECM) coating materials (laminin, fibronectin, Matrigel, and collagen IV) and the presence or absence of growth factors (EGF, bFGF, and NGF) in cell culture on the ultimate properties of EVs. The data show that OPC EVs and iOL EVs performed similarly to NPSC EVs in PC-12 proliferation and RAW264.7 mouse macrophage antiinflammatory assays, but NPSC EVs performed better in a PC-12 neurite outgrowth assay. Additionally, the presence of nerve growth factor (NGF) in culture was found to be maximize NPSC EV bioactivity among the conditions tested. NPSC EVs produced under rationally-selected culture conditions (fibronectin + NGF) enhanced axonal regeneration and muscle reinnervation in a rat nerve crush injury model. These results highlight the impact of culture conditions on NPSC EV neuroregenerative bioactivity, thus providing additional rationale for standardization and optimization of culture conditions for NPSC EV production. Table of Contents O_FIG O_LINKSMALLFIG WIDTH=200 HEIGHT=35 SRC="FIGDIR/small/528366v1_ufig1.gif" ALT="Figure 1"> View larger version (13K): org.highwire.dtl.DTLVardef@447d23org.highwire.dtl.DTLVardef@73a5cborg.highwire.dtl.DTLVardef@89dbb0org.highwire.dtl.DTLVardef@b62d39_HPS_FORMAT_FIGEXP M_FIG C_FIG Extracellular vesicles (EVs) purified from neural progenitor/stem cells (NPSCs) have been investigated for neurotherapeutic activity, however significant variability in culture conditions limits reproducibility and efficacy of this approach. Here, we examined the impact of extracellular matrix (ECM) components and growth factors in NPSC culture on the bioactivity on the bioactivity of their EVs. The results show that EVs from NPSCs cultured with a rationally-selected ECM type (fibronectin) and growth factor (nerve growth factor (NGF)) enhanced nerve regeneration and muscle recovery in a rat sciatic nerve crush injury model.

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Dutta, D., Pirolli, N. H., Levy, D., Tsao, J., Seecharan, N., Xu, X., Wang, Z., Jia, X., Jay, S. M.. 2023-02-15. Differentiation state and culture conditions impact neural stem/progenitor cell-derived extracellular vesicle bioactivity. https://doi.org/10.1101/2023.02.15.528366

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