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bioRxiv · 10.1101/2023.02.13.528383

NeoTCR: an immunoinformatic database of experimentally-supported functional neoantigen-specific TCR sequences

Zhou, W.·Xiang, W.·Yu, J.·Ruan, Z.·Pan, Y.·Wang, K.·Liu, J.
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Abstract

Neoantigen-based immunotherapy has demonstrated of long-lasting antitumor activity. Recognition of neoantigens by T cell receptors (TCRs) is considered a trigger for antitumor responses. Due to the overwhelming number of TCR repertoires in the human genome, it is challenging to computationally pinpoint neoantigen-specific TCRs. Recent studies have identified a number of functional neoantigen-specific TCRs, but the corresponding information is scattered across published literature and is difficult to retrieve. To improve access to these data, we developed the NeoTCR, an immunoinformatic database containing a unified description of publicly available neoantigen-specific TCR sequences, as well as relevant information on targeted neoantigens, from experimentally supported studies across 18 cancer subtypes. A user-friendly web interface allows interactive browsing and running of complex database queries based on numerous criteria. To facilitate rapid identification of neoantigen-specific TCRs from raw sequencing data, NeoTCR offers a one-stop analysis for annotation and visualization of TCR clonotypes, discovery of existing neoantigen-specific TCRs, and exclusion of bystander viral-associated TCRs. NeoTCR will serve as a valuable platform to study the biological functions of neoantigen-associated T-cells in anti-tumor immunity to better apply neoantigen-specific TCRs in clinics. NeoTCR is available at http://www.neotcrdb.com/.

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Zhou, W., Xiang, W., Yu, J., Ruan, Z., Pan, Y., Wang, K., Liu, J.. 2023-02-15. NeoTCR: an immunoinformatic database of experimentally-supported functional neoantigen-specific TCR sequences. https://doi.org/10.1101/2023.02.13.528383

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