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bioRxiv · 10.1101/2023.01.18.524628

Gravitational and mechanical forces drive mitochondrial translation through the cell adhesion-FAK axis

Abstract

Life on Earth has evolved in a form suitable for the gravitational force of 1 x g. Although the pivotal role of gravity in gene expression has been revealed by multiomics approaches in space-flown samples and astronauts, the molecular details of how mammalian cells harness gravity have remained unclear. Here, we showed that mitochondria utilize gravity to activate protein synthesis within the organelle. Genome-wide ribosome profiling revealed reduced mitochondrial translation in mammalian cells and Caenorhabditis elegans under both microgravity at the International Space Station and simulated microgravity in a 3D-clinostat on the ground. We found that attenuation of cell adhesion through laminin-integrin interactions causes the phenotype. The downstream signaling pathway including FAK, RAC1, PAK1, BAD, and Bcl-2 family proteins in the cytosol, and mitochondrial fatty acid synthesis (mtFAS) pathway in the matrix maintain mitochondrial translation at high level. Mechanistically, a decreased level of mitochondrial malonyl-CoA, which is consumed by activated mtFAS, leads to a reduction in the malonylation of the translational machinery and an increase in the initiation and elongation of in organello translation. Consistent with the role of integrin as a mechanosensor, we observed a decrease in mitochondrial translation via the minimization of mechanical stress in mouse skeletal muscle. Our work provides mechanistic insights into how cells convert gravitational and mechanical forces into translation in an energy-producing organelle.

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BibTeXRIS

Wakigawa, T., Kimura, Y., Mito, M., Tsubaki, T., Saito, H., Khan, A. H., Yamamori, T., Yamazaki, T., Higashibata, A., Tsuboi, T., Saito, T., Higashitani, A., Shichino, Y., Iwasaki, S.. 2023-01-19. Gravitational and mechanical forces drive mitochondrial translation through the cell adhesion-FAK axis. https://doi.org/10.1101/2023.01.18.524628

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