Molecular Mechanocytometry Using Tension-activated Cell Tagging (TaCT)

Ma, R.; Velusamy, A.; Rashid, A.; Deal, B. R.; Chen, W.; Petrich, B.; Li, R.; Salaita, K.

doi:10.1101/2023.01.10.523449

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bioRxiv · 10.1101/2023.01.10.523449

Molecular Mechanocytometry Using Tension-activated Cell Tagging (TaCT)

Ma, R.·Velusamy, A.·Rashid, A.·Deal, B. R.·Chen, W.·Petrich, B.·Li, R.·Salaita, K.

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Abstract

Flow cytometry is routinely used to measure single-cell gene expression by staining cells with fluorescent antibodies and nucleic acids. Here we present Tension-activated Cell Tagging (TaCT) to fluorescently label cells based on the magnitude of molecular force transmitted through cell adhesion receptors. As a proof-of-concept, we analyzed fibroblasts and mouse platelets after TaCT using conventional flow cytometry.

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Ma, R., Velusamy, A., Rashid, A., Deal, B. R., Chen, W., Petrich, B., Li, R., Salaita, K.. 2023-01-11. Molecular Mechanocytometry Using Tension-activated Cell Tagging (TaCT). https://doi.org/10.1101/2023.01.10.523449

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