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bioRxiv · 10.1101/202259

Spatially organizing biochemistry: choosing a strategy to translate synthetic biology to the factory

Jakobson, C.·Tullman-Ercek, D.·Mangan, N.
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Abstract

Natural biochemical systems are ubiquitously organized both in space and time. Engineering the spatial organization of biochemistry has emerged as a key theme of synthetic biology, with numerous technologies promising improved biosynthetic pathway performance. One strategy, however, may produce disparate results for different biosynthetic pathways. We propose a spatially resolved kinetic model to explore this fundamental design choice in systems and synthetic biology. We predict that two example biosynthetic pathways have distinct optimal organization strategies that vary based on pathway-dependent and cell-extrinsic factors. Moreover, we outline this design space in general as a function of kinetic and biophysical properties, as well as culture conditions. Our results suggest that organizing biosynthesis has the potential to substantially improve performance, but that choosing the appropriate strategy is key. The flexible mathematical framework we propose can be adapted to diverse biosynthetic pathways, and lays a foundation to rationally choose organization strategies for biosynthesis.

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Jakobson, C., Tullman-Ercek, D., Mangan, N.. 2017-10-12. Spatially organizing biochemistry: choosing a strategy to translate synthetic biology to the factory. https://doi.org/10.1101/202259

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