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bioRxiv · 10.1101/2022.12.26.521955

The interplay between adsorption and aggregation of von Willebrand factor chains in shear flows

Amaya-Espinosa, H.·Alexander-Katz, A.·Aponte-Santamaria, C.
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Abstract

AO_SCPLOWBSTRACTC_SCPLOWVon Willebrand factor (VWF) is a giant extracellular glycoprotein that carries out a key adhesive function during primary hemostasis. Upon vascular injury and triggered by the shear of flowing blood, VWF establishes specific interactions with several molecular partners in order to anchor platelets to collagen on the exposed sub-endothelial surface. VWF also interacts with itself to form aggregates that, adsorbed on the surface, provide more anchor sites for the platelets. However, the interplay between elongation and subsequent exposure of cryptic binding sites, self-association, and adsorption on the surface, remained unclear for VWF. In particular, the role of shear flow in these three processes is not well understood. In this study, we address these questions by using Brownian dynamics simulations at a coarse-grained level of resolution. We considered a system consisting of multiple VWF-like self-interacting chains that also interact with a surface under a shear flow. By a systematic analysis, we reveal that chain-chain and chain-surface interactions coexist non-trivially to modulate the spontaneous adsorption of VWF and the posterior immobilization of secondary tethered chains. Accordingly, these interactions tune VWFs extension and its propensity to form shear-assisted functional adsorbed aggregates. Our data highlights the collective behavior VWF self-interacting chains have when bound to the surface, distinct from that of isolated or flowing chains. Furthermore, we show that the extension and the exposure to solvent have a similar dependence on shear flow, at a VWF-monomer level of resolution. Overall, our results highlight the complex interplay that exists between adsorption, cohesion, and shear forces and its relevance for the adhesive hemostatic function of VWF.

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Amaya-Espinosa, H., Alexander-Katz, A., Aponte-Santamaria, C.. 2022-12-27. The interplay between adsorption and aggregation of von Willebrand factor chains in shear flows. https://doi.org/10.1101/2022.12.26.521955

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