High robustness of cytosolic glutathione redox potential under combined salt and osmotic stress in barley as revealed by the biosensor Grx1-roGFP2

Bohle, F.; Klaus, A.; Tegethof, H.; Schwarzländer, M.; Hochholdinger, F.; Meyer, A. J.; Acosta, I.; Müller-Schüssele, S. J.

doi:10.1101/2022.12.22.521445

Home/Archive/bioRxiv

bioRxiv · 10.1101/2022.12.22.521445

High robustness of cytosolic glutathione redox potential under combined salt and osmotic stress in barley as revealed by the biosensor Grx1-roGFP2

Bohle, F.·Klaus, A.·Tegethof, H.·Schwarzländer, M.·Hochholdinger, F.·Meyer, A. J.·Acosta, I.·Müller-Schüssele, S. J.

View original source ↗Export metadata ↓

Abstract

O_LIBarley is a staple crop of major global importance and relatively resilient to a wide range of stress factors in the field. Transgenic reporter lines to investigate physiological parameters during stress treatments remain scarce. C_LIO_LIWe generated and characterized stable homozygous barley lines (cv. Golden Promise Fast) expressing the genetically-encoded biosensor Grx1-roGFP2, which indicates the redox potential of the major antioxidant glutathione in the cytosol. C_LIO_LIOur results demonstrate functionality of the sensor in living barley plants. We determined the glutathione redox potential (EGSH) of the cytosol to be in the range of -308 to -320 mV. EGSH was robust against a combined NaCl (150 mM) and water deficit treatment (-0.8 MPa) that caused growth retardation and showed only a minor oxidation after 96 h of treatment. C_LIO_LIWe conclude that the generated reporter lines are a novel resource to study stress resilience in barley. C_LI One sentence summaryGeneration and characterization of barley plants stably expressing Grx1-roGFP2 reveal high robustness of cytosolic glutathione redox potential (EGSH) under combined salt and osmotic stress.

Explore related subjects

plant biology Preprint

Keep this discovery

Explore connections, maps & timelines

BibTeX RIS

Bohle, F., Klaus, A., Tegethof, H., Schwarzländer, M., Hochholdinger, F., Meyer, A. J., Acosta, I., Müller-Schüssele, S. J.. 2022-12-22. High robustness of cytosolic glutathione redox potential under combined salt and osmotic stress in barley as revealed by the biosensor Grx1-roGFP2. https://doi.org/10.1101/2022.12.22.521445

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Large seed size accompanying GmSDP1 suppression is linked to cellular remodeling and phytohormone regulation

Soybean (Glycine max) seeds contain approximately 20% oil and 40% protein and are important for food, feed, and biofuel. Improving soybean productivity and seed quality requires optimizing carbon partitioning during seed development. Previously, seed-specific suppression of the lipase-encoding gene Sugar-Dependent 1 (SDP1) increased seed oil content by approximately 5% while slightly reducing raffinose family oligosaccharides without compromising protein content. Seed-specific SDP1 suppression also increased seed size by 18-25% without affecting seed number, resulting in greater seed biomass per plant. To determine the developmental basis of the increased seed size, we investigated the cellular and molecular mechanisms underlying this phenotype. Microscopy and biochemical analyses showed that larger seeds were associated with increased seed cell size and enhanced lignocellulosic cell wall accumulation. Temporal biochemical and transcriptomic analyses further revealed coordinated changes in hormone homeostasis, including altered levels of abscisic acid, 12-oxo-phytodienoic acid, and jasmonoyl-isoleucine, together with differential expression of F-box and RING/U-box family genes. The results indicate that the enhanced seed size resulting from SDP1 suppression is associated with coordinated changes in cellular development, cell wall accumulation, hormone homeostasis, and regulation of gene expression during seed development. Developmental and metabolic changes associated with the increased seed size phenotype support SDP1 manipulation as a potential strategy for improving soybean seed yield and quality.

plant biology↗

bioRxiv2026

Growth, yield, and quality of hot pepper (Capsicum annuum L.) as affected by the combined application of liquid bio-slurry and NPS fertilizer rates in Kucha, Gamo zone, south Ethiopia

Hot pepper (Capsicum annuum L.) is a key cash and nutritional crop in Ethiopia, yet its productivity remains constrained by inadequate and imbalanced soil nutrient management. Therefore, the field experiment was conducted in Kucha woreda, Gamo Zone, South Ethiopia, during the 2024/25 cropping season to investigate the effect of liquid bio-slurry and NPS fertilizer rates on growth, yield and quality of hot pepper. The experiment consisted of 16 treatments arranged in a factorial combination of four liquid bio-slurry (LBS) rates (0, 20, 40, and 60 m3 ha-1) and four NPS fertilizer rates (0, 75, 150, and 225 kg ha-1) using a randomized complete block design with three replications. All experimental data were collected following standard agronomic procedures and subjected to analysis of variance using GenStat statistical software (18th edition). The results revealed that both the main and interaction effects of liquid bio-slurry and NPS significantly (p < 0.01) influenced phenological, growth, yield, and quality parameters. The combined application of 150 kg ha-1 NPS and 60 m3 ha-1 LBS produced the highest marketable dry fruit yield (2.94 t ha-1) and total dry fruit yield (3.16 t ha-1), representing a 129.69% yield advantage over the control (1.28 t ha-1) in the case of marketable dry fruit yield. The findings demonstrate that integrated nutrient management using combined LBS and NPS fertilizers significantly enhanced the productivity and quality of hot pepper. Therefore, the application of 150 kg ha-1 NPS combined with 60 m3 ha-1 liquid bio-slurry is recommended for improved hot pepper production for the study area and similar agro-ecological conditions.

plant biology↗

bioRxiv2026

Higher stomatal density and smaller stomatal size associate with higher grain yield in winter wheat

In wheat (Triticum aestivum L.), flag leaf is crucial in determining grain-filling rate and final grain yield. Stomata, microscopic pores on plant surfaces, balance CO2 uptake and evaporative water loss; their numbers and aperture sizes depend on environmental factors. Previous studies have reported associations between stomatal traits and grain yield; mostly under controlled conditions. Our study aimed to investigate flag leaf stomatal anatomical and physiological traits, and their relationships with winter wheat grain yield under field conditions. We measured stomatal density and size, and stomatal conductance on adaxial and abaxial flag leaf surfaces in a three-year field study with twenty-five winter wheat varieties. Stomatal density ratio remained constant despite different stomatal densities occurring in different years, indicating coordinated adjustments of stomatal numbers between leaf surfaces in response to environmental conditions. Stomatal conductance ratios varied widely, indicating leaf-surface specific stomatal responses to environmental conditions. Grain yield was positively associated with stomatal density and negatively with stomatal size. Flag leaf removal reduced grain weight per spike and did not affect grain numbers per spike, unlike in spring wheat. Our field study provides valuable insights into the relationships between winter wheat stomatal traits and grain yield that can be used in breeding.

plant biology↗