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bioRxiv · 10.1101/2022.12.15.520561

SARS-CoV-2 causes periodontal fibrosis by deregulating mitochondrial beta-oxidation

Gao, Y.·Kok, W. L.·Sharma, V.·Illsley, C. S.·Hanks, S.·Tredwin, C.·Hu, B.
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Abstract

The global high prevalence of COVID-19 is a major challenge for health professionals and patients. SARS-CoV-2 virus mutate predominantly in the spike proteins, whilst the other key viral components remain stable. Previous studies have shown that the human oral cavity can potentially act as reservoir of the SARS-CoV-2 virus. COVID-19 can cause severe oral mucosa lesions and is likely to be connected with poor periodontal conditions. However, the consequence of SARS-CoV-2 viral infection on human oral health has not been systematically examined. In this research, we aimed to study the pathogenicity of SARS-CoV-2 viral components on human periodontal tissues and cells. We found that by exposing to SARS-CoV-2, especially to the viral envelope and membrane proteins, the human periodontal fibroblasts could develop fibrotic pathogenic phenotypes, including hyperproliferation that was concomitant induced together with increased apoptosis and senescence. The fibrotic degeneration was mediated by a down-regulation of mitochondrial {beta}-oxidation in the fibroblasts. Fatty acid {beta}-oxidation inhibitor, etomoxir treatment could mirror the same pathological consequence on the cells, similar to SARS- CoV-2 infection. Our results therefore provide novel mechanistic insights into how SARS- CoV-2 infection can affect human periodontal health at the cell and molecular level with potential new therapeutic targets for COVID-19 induced fibrosis.

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Gao, Y., Kok, W. L., Sharma, V., Illsley, C. S., Hanks, S., Tredwin, C., Hu, B.. 2022-12-15. SARS-CoV-2 causes periodontal fibrosis by deregulating mitochondrial beta-oxidation. https://doi.org/10.1101/2022.12.15.520561

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