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bioRxiv · 10.1101/2022.12.14.520451

Monitoring the battlefield: Antimicrobial resistance, antibiofilm patterns and virulence factors of Acinetobacter baumannii isolates from hospital system

Awuor, S.
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Abstract

Hospital-acquired contagions are on the increase and are a substantial cause of clinical and financial burden for healthcare systems. While contagion control plays a major role in curtailing the spread of outbreak organisms, it is not always effective. One organism of particular concern is Acinetobacter baumannii, due to both its persistence in the hospital setting and its ability to acquire antibiotic resistance. A. baumannii has appeared as a nosocomial pathogen that displays high levels of resistance to antibiotics, and remains resilient against traditional cleaning measures with resistance to Colistin increasingly reported. Given the costs associated and magnitude with hospital acquired infections, and the rise in multidrug-resistant organisms, it is worth re-evaluating our current approaches and observing for substitutes or aides-de-camp to traditional antibiotics therapies. The aims of this study was to determine antimicrobial resistance, antibiofilm patterns and virulence factors of Acinetobacter baumannii isolates from hospital system. Among the five drugs used (Amikacin, Gentamicin, Meropenem, Tigecycline and Tazobactam) it was revealing that all the isolates show resistance to all the drugs. It was revealed that 3 isolates show resistance at difference antimicrobial classes in isolate 05/JOOTRH/22 and 17/JOOTRH/22 were able to produce all type of the virulence enzymes while isolate 11/JOOTRH/22 was able to produce all except protease enzyme. The isolates showed that the biofilm formation inhibitory effects of the various concentrations (0.5, 0.25, 0.125, 0.0625 and 0.03125 mg ml-1) were significantly lower than that of the positive control, an indication that biofilm formation was inhibited at these concentrations

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Awuor, S.. 2022-12-15. Monitoring the battlefield: Antimicrobial resistance, antibiofilm patterns and virulence factors of Acinetobacter baumannii isolates from hospital system. https://doi.org/10.1101/2022.12.14.520451

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