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bioRxiv · 10.1101/2022.12.13.520220

NanopoReaTA: a user-friendly tool for nanopore-seq real-time transcriptional analysis

Wierczeiko, A.·Pastore, S.·Muendnich, S.·Helm, M.·Butto, T.·Gerber, S.
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Abstract

SummaryOxford Nanopore Technologies (ONT) sequencing platform offers an excellent opportunity to perform real-time analysis during sequencing. This feature allows for early insights into experimental data and accelerates a potential decision-making process for further analysis, which can be particularly relevant in the clinical context. Although some tools for the real-time analysis of DNA-sequencing data already exist, there is currently no application available for differential transcriptome data analysis designed for scientists or physicians with limited bioinformatics knowledge. Here we introduce NanopoReaTA, a user-friendly real-time analysis toolbox for RNA sequencing data from ONT. Sequencing results from a running or finished experiment are processed through an R Shiny-based graphical user interface (GUI) with an integrated Nextflow pipeline for whole transcriptome or gene-specific analyses. NanopoReaTA provides visual snapshots of a sequencing run in progress, thus enabling interactive sequencing and rapid decision-making that could also be applied to clinical cases. Availabilityhttps://github.com/AnWiercze/NanopoReaTA Contactbuttamer@uni-mainz.de and sugerber@uni-mainz.de

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Wierczeiko, A., Pastore, S., Muendnich, S., Helm, M., Butto, T., Gerber, S.. 2022-12-15. NanopoReaTA: a user-friendly tool for nanopore-seq real-time transcriptional analysis. https://doi.org/10.1101/2022.12.13.520220

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