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bioRxiv · 10.1101/2022.12.11.519977

Site-directed placement of three-dimensional DNA origami

Martynenko, I. V.·Erber, E.·Ruider, V.·Dass, M.·Posnjak, G.·Yin, X.·Altpeter, P.·Liedl, T.
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Abstract

Assembling hybrid substrates with nanometer-scale precision and molecular addressability enables advances in such distant fields as material research and biosensing. As such, the combination of lithographic methods with 2D DNA origami self-assembly [1-4] has led, among others, to the development of photonic crystal cavity arrays [2] and the exploration of sensing nanoarrays where molecular devices are patterned on the sub-micron scale [5-7]. Here we extend this concept to the third dimension through mounting 3D DNA origami onto nano-patterned substrates followed by silicification [8, 9] to provide mechanical and chemical stability. Our versatile and scalable method relying on self-assembly at ambient temperatures offers the potential to 3D-position any inorganic and organic components that are compatible with DNA architectures [10-13]. This way, complex and 3D-patterend surfaces designed on the molecular level while reaching macroscopic dimensions could supersede energy-intensive manufacturing steps in substrate processing.

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Martynenko, I. V., Erber, E., Ruider, V., Dass, M., Posnjak, G., Yin, X., Altpeter, P., Liedl, T.. 2022-12-13. Site-directed placement of three-dimensional DNA origami. https://doi.org/10.1101/2022.12.11.519977

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