Cell surface auxin signalling directly targets PIN-mediated auxin fluxes for adaptiveplant development

Rodriguez, L.; Fiedler, L.; Zou, M.; Giannini, C.; Monzer, A.; Gelova, Z.; Verstraeten, I.; Hajny, J.; Tan, S.; Hoermayer, L.; Li, L.; Marques-Bueno, M. M.; Molnar, G.; Xu, T.; Kulich, I.; Jaillais, Y.; Friml, J.

doi:10.1101/2022.11.30.518503

bioRxiv · 10.1101/2022.11.30.518503

Cell surface auxin signalling directly targets PIN-mediated auxin fluxes for adaptiveplant development

Rodriguez, L.·Fiedler, L.·Zou, M.·Giannini, C.·Monzer, A.·Gelova, Z.·Verstraeten, I.·Hajny, J.·Tan, S.·Hoermayer, L.·Li, L.·Marques-Bueno, M. M.·Molnar, G.·Xu, T.·Kulich, I.·Jaillais, Y.·Friml, J.

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Abstract

Phytohormone auxin and its directional transport mediate much of the remarkably plastic development of higher plants. Positive feedback between auxin signaling and transport is a key prerequisite for (i) self-organizing processes including vascular tissue formation and (ii) directional growth responses such as gravitropism. Here we identify a mechanism, by which auxin signaling directly targets PIN auxin transporters. Via the cell-surface ABP1-TMK1 receptor module, auxin rapidly induces phosphorylation and thus stabilization of PIN2. Following gravistimulation, initial auxin asymmetry activates autophosphorylation of the TMK1 kinase. This induces TMK1 interaction with and phosphorylation of PIN2, stabilizing PIN2 at the lower root side, thus reinforcing asymmetric auxin flow for root bending. Upstream of TMK1 in this regulation, ABP1 acts redundantly with the root-expressed ABP1-LIKE auxin receptor ABL3. Such positive feedback between cell-surface auxin signaling and PIN-mediated polar auxin transport is fundamental for robust root gravitropism and presumably also for other self-organizing developmental phenomena.

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Rodriguez, L., Fiedler, L., Zou, M., Giannini, C., Monzer, A., Gelova, Z., Verstraeten, I., Hajny, J., Tan, S., Hoermayer, L., Li, L., Marques-Bueno, M. M., Molnar, G., Xu, T., Kulich, I., Jaillais, Y., Friml, J.. 2022-11-30. Cell surface auxin signalling directly targets PIN-mediated auxin fluxes for adaptiveplant development. https://doi.org/10.1101/2022.11.30.518503

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