In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

Kakuk, B.; Dormo, A.; Csabai, Z.; Kemenesi, G.; Holoubek, J.; Ruzek, D.; Prazsak, I.; Dani, V. E.; Denes, B.; Torma, G.; Jakab, F.; Toth, G. E.; Foldes, F. V.; Zana, B.; Lanszki, Z.; Harangozo, A.; Fulop, A.; Gulyas, G.; Mizik, M.; Kiss, A. A.; Tombacz, D.; Boldogkoi, Z.

doi:10.1101/2022.11.28.518226

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bioRxiv · 10.1101/2022.11.28.518226

In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing

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Abstract

The recent Monkeypox outbreak showed the importance of studying the basic biology of orthopoxviruses. However, the transcriptome of its causative agent has not been investigated before neither with short-, nor with long-read sequencing approaches. This Oxford Nanopore long-read RNA-Sequencing dataset fills this gap. Our direct cDNA and native RNA sequencing data enable the in-depth characterization of the transcriptomic architecture and dynamics of the gene expressions of monkeypox virus; and also the deeper understanding of the changes it causes in the host cells on a transcriptome level.

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Kakuk, B., Dormo, A., Csabai, Z., Kemenesi, G., Holoubek, J., Ruzek, D., Prazsak, I., Dani, V. E., Denes, B., Torma, G., Jakab, F., Toth, G. E., Foldes, F. V., Zana, B., Lanszki, Z., Harangozo, A., Fulop, A., Gulyas, G., Mizik, M., Kiss, A. A., Tombacz, D., Boldogkoi, Z.. 2022-12-02. In-depth Temporal Transcriptome Profiling of Monkeypox and Host Cells using Nanopore Sequencing. https://doi.org/10.1101/2022.11.28.518226

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