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bioRxiv · 10.1101/2022.11.27.518129

Conformational changes in the essential E. coli septal cell wall synthesis complex suggest an activation mechanism

Britton, B. M.·Yovanno, R. A.·Costa, S. F.·McCausland, J.·Lau, A. Y.·Xiao, J.·Hensel, Z.
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Abstract

The bacterial divisome, a macromolecular machine that is composed of more than thirty proteins in E. coli, orchestrates the essential process of cell wall constriction during cell division. Novel antimicrobial strategies can target protein-protein interactions within the divisome and will benefit from insights into divisome structure and dynamics. In this work, we combined structure prediction, molecular dynamics simulation, single-molecule imaging, and mutagenesis to construct a model of the core complex of the E. coli divisome composed of the essential septal cell wall synthase complex formed by FtsW and FtsI, and its regulators FtsQ, FtsL, FtsB, and FtsN. We observed extensive interactions in four key regions in the periplasmic domains of the complex. FtsQ, FtsL, and FtsB scaffold FtsI in an extended conformation with the FtsI transpeptidase domain lifted away from the membrane through interactions among the C-terminal domains. FtsN binds between FtsI and FtsL in a region rich in residues with superfission (activating) and dominant negative (inhibitory) mutations. Mutagenesis experiments in cellulo and in silico revealed that the essential domain of FtsN functions as a tether to tie FtsI and FtsL together, impacting interactions between the anchor-loop of FtsI and the putative catalytic region of FtsW, suggesting a mechanism of how FtsN activates the cell wall synthesis activities of FtsW and FtsI.

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Britton, B. M., Yovanno, R. A., Costa, S. F., McCausland, J., Lau, A. Y., Xiao, J., Hensel, Z.. 2022-11-28. Conformational changes in the essential E. coli septal cell wall synthesis complex suggest an activation mechanism. https://doi.org/10.1101/2022.11.27.518129

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