Precision RNAi using synthetic shRNAmir target sites

Hoffmann, T.; Hörmann, A.; Corcokovic, M.; Zmajkovic, J.; Hinterndorfer, M.; Salkanovic, J.; Spreitzer, F.; Köferle, A.; Gitschtaler, K.; Popa, A.; Oberndorfer, S.; Andersch, F.; Schaefer, M.; Fellner, M.; Budano, N.; Ruppert, J. G.; Chetta, P.; Wurm, M.; Zuber, J.; Neumüller, R. A.

doi:10.1101/2022.11.24.517802

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bioRxiv · 10.1101/2022.11.24.517802

Precision RNAi using synthetic shRNAmir target sites

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Abstract

Loss-of-function genetic tools are widely applied for validating therapeutic targets, but their utility remains limited by incomplete on- and uncontrolled off-target effects. We describe artificial RNA interference (ARTi) based on synthetic, ultra-potent, off-target-free shRNAs that enable efficient and inducible suppression of any gene upon introduction of a synthetic target sequence into non-coding transcript regions. ARTi establishes a scalable loss-of-function tool with full control over on- and off-target effects.

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Hoffmann, T., Hörmann, A., Corcokovic, M., Zmajkovic, J., Hinterndorfer, M., Salkanovic, J., Spreitzer, F., Köferle, A., Gitschtaler, K., Popa, A., Oberndorfer, S., Andersch, F., Schaefer, M., Fellner, M., Budano, N., Ruppert, J. G., Chetta, P., Wurm, M., Zuber, J., Neumüller, R. A.. 2022-11-25. Precision RNAi using synthetic shRNAmir target sites. https://doi.org/10.1101/2022.11.24.517802

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