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bioRxiv · 10.1101/2022.11.24.517799

Fake IDs? Widespread misannotation of DNA Transposons as a General Transcription Factor

Hassan, N. T.·Adelson, D. L.
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Abstract

The annotation of transposable elements (TEs) is a critical part of our understanding of genomes, however, the accuracy of annotation pipelines remains an issue as TEs are frequently underestimated or misannotated. We report the General Transcription Factor II-I Repeat Domain-Containing Protein 2 (GTF2IRD2) was used to erroneously annotate DNA transposons in a variety of non-mammalian species as GTF2IRD2 contains a 3 fused hAT transposase domain. This study emphasises that the misannotation of TEs as trans-regulatory elements, such as transcription factors, can lead to errors in phylogenetic trees based on orthologs and to significant wasted time for investigators interested in gene regulation and characterising non-mammalian genomes.

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Hassan, N. T., Adelson, D. L.. 2022-11-24. Fake IDs? Widespread misannotation of DNA Transposons as a General Transcription Factor. https://doi.org/10.1101/2022.11.24.517799

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