bioRxiv Science⌕ Search
Home/Archive/bioRxiv

bioRxiv · 10.1101/2022.11.22.517544

CYTOKINE-BASED MULTI-EPITOPE PROTEIN FOR BINDING TO CCR7-POSITIVE CELLS

Beihaghi, m. B.·Beihaghi, M.·Marashi, H.
View original source ↗Export metadata ↓

Abstract

Drugs based on cytokine genes increase the bodys immunity against cancer and several viral diseases. Cytokines such as CCL21 and CCL19 specifically bind to the CCR7 receptor and have anti-tumor properties and a prognosis of tumorigenesis. An epitope of IL-I{beta} is also involved in various cellular activities such as neutrophil activation, T and B lymphocyte cell production, antibody production, and fibroblast proliferation. They bind to secretory proteins without any inflammatory response. GM-CSF adjuvant is one of the growth factors of white blood cells (WBC) that stimulates stem cells to produce granulocytes and myocytes. In our project, we designed and synthesized codon-optimized multiepitope genes construct from human genes. The molecular dynamics (MD) simulation and binding affinity of this recombinant protein and CCR7 receptor were examined through in silico analyses. This construct was introduced into the pET-28a vector that was cloned in Ecoli to explore this recombinant protein. The purified multiepitope protein produced a strong signal in Dot-blot, SDS-PAGE, and Western-blot assays comparable to the positive control. The assessments of FTIR measurement and MALDI-TOF MS displayed that synthetic gene constructs correctly be expressed in E. coli. We also investigated the potential activity of the purified multiepitope protein in stimulating migration and proliferation of MCF7CCR7+ cancer cell line using wound healing assay. Also, in this study, the MTT method was used to determine the half-maximum inhibitory concentration (IC50) of all multiepitope protein concentrations. We used Agarose assay on PBMCsCCR7+ to observe whether PBMC cells and chemoattractants attract to each other. Also, we found that intraperitoneal injection of recombinant antigen affected the level of WBC in BALB/c mice, and the level of WBC in tumor mice increased significantly compared to healthy mice. Our project aims to produce the first multi-epitope vaccine with many beneficial advantages such as low-cost price and any major or significant complications that can be used as biomarkers for cancer screening and prognosis tests to immunize the patient before chemotherapy.

Explore related subjects

bioengineeringPreprint

Keep this discovery

Explore connections, maps & timelines

BibTeXRIS

Beihaghi, m. B., Beihaghi, M., Marashi, H.. 2022-11-24. CYTOKINE-BASED MULTI-EPITOPE PROTEIN FOR BINDING TO CCR7-POSITIVE CELLS. https://doi.org/10.1101/2022.11.22.517544

Cite the original work for its findings. Save a collection to share your selection of sources.

KEEP EXPLORING

Related preprints

bioRxiv2026

Comparative study of chlorophyll measurement in Physcomitrium patens moss using a conventional microscope adapted for combined 2D+1D imaging and spectral analysis

Imaging spectroscopy often requires expensive and complex equipment. Here we show a simple procedure for attaching a standard miniature fiber spectrometer to a conventional microscope, allowing easy integration of 2D imaging with 1D high-resolution spectral measurements. This combination provides much of the benefit of a full imaging spectrometer without the large equipment investment, and we provide instructions for modifying microscopes to this setup and the present measurements of living cells that demonstrate their performance. Using this setup, we compare the quantitative measurement of chlorophyll concentration in Physcomitrium patens moss using color imaging and spectral sampling.

bioengineering↗
bioRxiv2026

De novo designed single-domain antibodies protect against lethal cobra venom neurotoxicity in vivo

Generative protein design can now rapidly produce de novo binders with high affinity and functional activity against a wide range of targets, including lethal snake venom toxins. However, so far most reported successes rely on new-to-nature scaffolds with limited therapeutic precedent. Single-domain antibodies (VHHs) offer a clinically validated alternative scaffold that can bind and neutralize long-chain -neurotoxins, which are some of the most lethal components in snake venoms. Here we compare three recently established de novo design models with VHH-design capabilities (Germinal, RFantibody, and BoltzGen) for their ability to generate VHHs against the neurotoxin -cobratoxin from the monocled cobra (Naja kaouthia). Using standardized model inputs and evaluation criteria based on AlphaFold3 interface confidence (ipTM) and RMSD self-consistency, we find that Germinal was the only method to generate designs passing stringent in silico criteria for experimental testing. We therefore performed a larger Germinal design campaign employing three different VHH frameworks and experimentally validated 46 designs in vitro. Of these, 42 expressed as soluble proteins and we identified four binding hits derived from two of the three tested frameworks. Of the four binders, two lead candidates were further characterized and demonstrated high affinity (KDs of 4.1 nM and 10.8 nM), monomeric behavior and low polyreactivity, indicating favorable biophysical and developability properties, as well as functional toxin neutralization in vitro. To assess their therapeutic potential we investigated their ability to protect against -cobratoxin toxicity in vivo. Both candidates fully protected mice after -cobratoxin challenge, with 100% survival compared to a lethal control. One candidate also retained notable neutralization capacity against whole venom of Naja kaouthia with a survival of 56%, while the other protected 22% when tested in a rescue setting. Together, we demonstrate that de novo VHH design can generate high affinity single-domain antibodies with in vivo protection against lethal cobra venom neurotoxicity, and provide practical insights into method- and framework-dependent performance.

bioengineering↗
bioRxiv2026

Simple Feedback for Complex Movement: Capturing Whole-Limb Reorganization during Single-IMU Gait Retraining

Clinical gait retraining typically relies on multi-sensor arrays and high-dimensional feedback displays, imposing setup and interpretation burdens that limit routine clinical deployment. We developed a single-IMU visual biofeedback system that delivers real-time feedback of Lower Limb Trajectory Error (LLTE), a composite kinematic error metric integrating knee position and shank angle across the stance phase. Twenty able-bodied adults walked on a treadmill under two visual biofeedback targets (flexed-knee, extended-knee) while receiving either corrected (n=10) or uncorrected (n=8) feedback, where the correction accounted for limb orientation at initial contact. LLTE and stance-phase knee kinematics adapted consistently under the flexed-knee target for both feedback groups, with feedback formulation moderating the temporal trajectory of change. Adaptation toward the extended-knee target was limited, likely because participants were already operating near terminal knee extension and because the scalar error metric provided limited directional information for correction. Ankle range of motion (ROM) changed significantly across the stance phase under both target conditions, while hip ROM did not. Multiscale multivariate sample entropy (MSMVSE) increased monotonically with time scale across all conditions, with no statistically distinguishable difference between corrected and uncorrected feedback. These results suggest that single-IMU LLTE biofeedback can modify gait mechanics and that adaptation was expressed across multiple lower-limb segments rather than through changes at a single joint.

bioengineering↗