Urine proteomic analysis of the rat startle model

Zhao, c.; Liu, Y.; Gao, Y.

doi:10.1101/2022.11.19.517176

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bioRxiv · 10.1101/2022.11.19.517176

Urine proteomic analysis of the rat startle model

Zhao, c.·Liu, Y.·Gao, Y.

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Abstract

Urine proteomics was applied to explore whether the effect of startle can be detected in urine. A combination of natural enemy odor and sound stimulation was used to establish the rat startle model. Urine samples were collected before and after startle, and urine proteomes before-after startle of each rat were compared individually. Regulatory subunits of glutamate-cysteine ligase was identified as the sole differential protein among all five startled rats. To our surprise, its functional partner catalytic subunits of glutamate-cysteine ligase was also identified in four out of five rats as differential protein. When comparing before-after startle as two groups, 22 differential proteins were identified which represent biological pathways including neurotransmitter transport and glucose transmembrane transport.

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Zhao, c., Liu, Y., Gao, Y.. 2022-11-19. Urine proteomic analysis of the rat startle model. https://doi.org/10.1101/2022.11.19.517176

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