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bioRxiv · 10.1101/2022.11.18.517062

Finding Consensus miRNAs Silencing KLF1 Expression as A Promising Therapeutic Option of Sickle Cell Anemia

Al-Madhagi, H. A.
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Abstract

1Sickle cell anemia (SCA) is the almost the severest hemoglobinopathy known with no cure till date. Patients with SCA has a shorter lifespan and suffer from painful crises and end-organ damages. The goal of this in silico work is to find the consensus miRNAs targeting KLF1 gene, responsible for HbF-to-HbA switching followed by generating the corresponding miRNA sponge for gene silencing purposes. 3 publicly available databases were searched, miRDB, miRWalk and TargetScan. Afterwards, the hybridization examination of the predicted miRNAs was evaluated. Finally, the design of miRNA sponge as a means to target miRNA was performed. In conclusion, hsa-miR-330-5p was the best miRNA targeting KLF1 gene in many aspects and its miRNA sponge sequence was provided.

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Al-Madhagi, H. A.. 2022-11-18. Finding Consensus miRNAs Silencing KLF1 Expression as A Promising Therapeutic Option of Sickle Cell Anemia. https://doi.org/10.1101/2022.11.18.517062

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