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bioRxiv · 10.1101/2022.11.14.516499

Two-photon targeted, quad whole-cell patch clamping robot

Gonzalez, G. I. V.·Kgwarae, P. O.·Schultz, S. R.
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Abstract

We present an automated quad-channel patch-clamp technology platform for ex vivo brain slice electrophysiology, capable of both blind and two-photon targeted robotically automated patching. The robot scales up the patch-clamp singlecell recording technique to four simultaneous channels, with seal success rates for two-photon targeted and blind modes of 54% and 68% respectively. In 50% of targeted trials (where specific cells were required), at least 2 simultaneous recordings were obtained. For blind mode, most trials yielded dual or triple recordings. This robot, a milestone on the path to a true in vivo robotic multi-patching technology platform, will allow numerous studies into the function and connectivity patterns of both primary and secondary cell types.

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Gonzalez, G. I. V., Kgwarae, P. O., Schultz, S. R.. 2022-11-16. Two-photon targeted, quad whole-cell patch clamping robot. https://doi.org/10.1101/2022.11.14.516499

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