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bioRxiv · 10.1101/2022.11.12.516272

Contrasting effector profiles between bacterial colonisers of kiwifruit reveal redundant roles and interplay converging on PTI-suppression and RIN4

Jayaraman, J.·Yoon, M.·Hemara, L. M.·Bohne, D.·Tahir, J.·Chen, R.·Brendolise, C.·Rikkerink, E. H.·Templeton, M. D.
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Abstract

O_LITesting effector-knockout strains of the Pseudomonas syringae pv. actinidiae biovar 3 (Psa3) for reduced in planta growth in their native kiwifruit host revealed a number of non- redundant effectors that contribute to Psa3 pathogenicity. Conversely, complementation in the weak kiwifruit pathogen P. syringae pv. actinidifoliorum (Pfm) for increased growth identified redundant Psa3 effectors. C_LIO_LIPsa3 effectors hopAZ1a and HopS2b and the entire exchangeable effector locus ({Delta}EEL; 10 effectors) were significant contributors to bacterial colonisation of the host and were additive in their effects on pathogenicity. Four of the EEL effectors (HopD1a, AvrB2b, HopAW1a, and HopD2a) redundantly contribute to pathogenicity through suppression of pattern-triggered immunity (PTI). C_LIO_LIImportant Psa3 effectors include several redundantly required effectors early in the infection process (HopZ5a, HopH1a, AvrPto1b, AvrRpm1a, and HopF1e). These largely target the plant immunity hub, RIN4. C_LIO_LIThis comprehensive effector profiling revealed that Psa3 carries robust effector redundancy for a large portion of its effectors, covering a few functions critical to disease. C_LI

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Jayaraman, J., Yoon, M., Hemara, L. M., Bohne, D., Tahir, J., Chen, R., Brendolise, C., Rikkerink, E. H., Templeton, M. D.. 2022-11-13. Contrasting effector profiles between bacterial colonisers of kiwifruit reveal redundant roles and interplay converging on PTI-suppression and RIN4. https://doi.org/10.1101/2022.11.12.516272

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