Shadow imaging for panoptical visualisation of living brain tissue

Dembitskaya, Y.; Boyce, A. K. J.; Idziak, A.; Pourkhalili Langeroudi, A.; Le Bourdelles, G.; Girard, J.; Arizono, M.; Ducros, M.; Sato-Fitoussi, M.; Ochoa de Amezaga, A.; Oizel, K.; Bancelin, S.; Mercier, L.; Pfeiffer, T.; Thompson, R. J.; Kim, S. K.; Bikfalvi, A.; Nagerl, V.

doi:10.1101/2022.11.03.511028

bioRxiv · 10.1101/2022.11.03.511028

Shadow imaging for panoptical visualisation of living brain tissue

Dembitskaya, Y.·Boyce, A. K. J.·Idziak, A.·Pourkhalili Langeroudi, A.·Le Bourdelles, G.·Girard, J.·Arizono, M.·Ducros, M.·Sato-Fitoussi, M.·Ochoa de Amezaga, A.·Oizel, K.·Bancelin, S.·Mercier, L.·Pfeiffer, T.·Thompson, R. J.·Kim, S. K.·Bikfalvi, A.·Nagerl, V.

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Abstract

Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation, do not have cellular resolution or only give a fragmented view. Here, we show how regular light microscopy together with fluorescence labeling of the interstitial fluid in the extracellular space provide comprehensive optical access in real-time to the anatomical complexity and dynamics of living brain tissue.

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Dembitskaya, Y., Boyce, A. K. J., Idziak, A., Pourkhalili Langeroudi, A., Le Bourdelles, G., Girard, J., Arizono, M., Ducros, M., Sato-Fitoussi, M., Ochoa de Amezaga, A., Oizel, K., Bancelin, S., Mercier, L., Pfeiffer, T., Thompson, R. J., Kim, S. K., Bikfalvi, A., Nagerl, V.. 2022-11-03. Shadow imaging for panoptical visualisation of living brain tissue. https://doi.org/10.1101/2022.11.03.511028

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